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Image Search Results
Journal: Scientific Reports
Article Title: Neural tube opening and abnormal extraembryonic membrane development in SEC23A deficient mice
doi: 10.1038/srep15471
Figure Lengend Snippet: ( A ) Real-time RT-PCR quantification of select UPR genes in E9.5–E11.5 yolk sac and E11.5 amnion. Expression of the GAPDH gene was used as a reference. Data are mean ± SD. Asterisks indicate statistically significant difference between WT and Sec23a gt/gt samples (* P < 0.05, ** P < 0.01). ( B ) TUNEL staining (green) was performed for E11.5 amnion. Nuclei were visualized by DAPI staining (blue). TUNEL-positive cells were quantified as percentages of total cell numbers. ( C ) Activation of the PERK pathway in Sec23a gt/gt yolk sacs. Equal amounts of extracts from E11.5 yolk sac were immunoblotted for eIF2α, the phospohorylated eIF2α (p-eIF2α), GRP78 and β-actin.
Article Snippet: Other antibodies used include: mouse monoclonal anti-KDEL (MBL; 1:100), mouse monoclonal anti-PDI (Assay Designs; 1:200), rabbit polyclonal anti-Ki67 (Abcam; 1:500), rabbit polyclonal anti-fibronectin (Abcam; 1:100), mouse monoclonal anti-eIF2α and rabbit polyclonal anti-p-eIF2α (Cell Signaling; 1:1000),
Techniques: Quantitative RT-PCR, Expressing, TUNEL Assay, Staining, Activation Assay